Protocols

Our rat monoclonal antibodies are obtained in the form of hybridoma cell culture supernatants and these contain sodium azide (0.05% w/v) as a preservative. They can be stored at 4oC where they will remain active for many years. They can be frozen but repeated freeze/thaw cycles should be avoided.

The antibodies are used as dilutions of between 10-fold and 100-fold in PBS (phosphate-buffered saline) with a blocking protein such as milk protein or BSA. All secondary detection antibodies should also be diluted appropriately in this buffer

Each stage of antibody incubation should be carried out for at least 1 hour. Incubations are mostly done at RT but O/N incubations can be done at 4oC.

LM- and JIM-series monoclonal antibodies are RAT monoclonal antibodies and require anti-rat-IgG (whole molecule) secondary reagents.

New protocol using our antibodies/CBMs at bio-protocol

Cornuault V, Knox JP (2014) Sandwich ELISA analysis of plant cell wall glycan connections. bio-protocol ONLINE

Using rat monoclonal antibodies in...

Immunofluorescence labelling for light microscopy and immunogold labelling for electron microscopy
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Nitrocellulose-based assays (IDAs) and microtitre plate-based assays (ELISAs)
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