Monoclonal Antibodies

Monoclonal antibodies to plant cell wall polymers generated or maintained in our laboratory are detailed below.

PDF: Overview list and short guide to our antibody probes 

Antibody specificities and links to publications

Availability

Large selections of our rat MAbs are now available from Ximbio based in the UK, Megazyme (serum-free antibodies) based in Ireland and also Kerafast based in Boston, USA. All companies can ship worldwide.

The BAM- antibodies to brown alga fucans and alginates are available from SeaProbes in Roscoff.

Selections of JIM- and MAC-antibodies (to AGPs/extensins) are also available from Carbosource at the CCRC/University of Georgia.

The PlantProbes activity, that maintained the availability of monoclonal antibodies from our laboratory, was in operation from 1998 until it ceased distribution in June 2021. We are thankful for all the interest shown during this period.

Our cell wall antibodies are highly sensitive, highly specific and versatile molecular tools that can be used for microscopy analyses and quantitative assessments of cell wall polymers in all plant materials ranging from fruits and vegetable to fibres and all forms of plant biomass. We are happy to discuss any potential uses or applications of our probes.

Any enquiries about our antibodies can be addressed to Paul Knox 

Nomenclature

LM– Leeds Monoclonal, BAM– Brown Algal Monoclonal, JIM– John Innes Monoclonal, MAC– Monoclonal Antibody Centre (Babraham).

All antibodies listed here are rat monoclonal antibodies.
Reagents we recommend for their detection include:
Peroxidase-linked
Anti-rat IgG (whole molecule)-peroxidase produced in rabbit (Sigma A9542)
Fluorochromes
Anti-rat IgG (whole molecule)-FITC produced in rabbit (Sigma F1763)
Gold-linked
Anti-rat IgG (whole molecule)-Gold produced in goat (Sigma G7035)

Downloads - Antibody lists and Pectin cartoons

Downloads

List of monoclonal antibodies
JPKab2021

Cartoon depictions of pectic structures and epitopes
PectAb19 PDF